Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress

Traction Forces of Endothelial Cells under Slow Shear Flow.

Endothelial cells are constantly exposed to fluid shear stresses that regulate vascular morphogenesis, homeostasis, and disease. The mechanical responses of endothelial cells to relatively high shear flow such as that characteristic of arterial circulation has been extensively studied. Much less is known about the responses of endothelial cells to slow shear flow such as that characteristic of ...

Gene expression profiling of human aortic endothelial cells exposed to disturbed flow and steady laminar flow.

Subtraction cloning and cDNA arrays were used to compare steady-state mRNA levels in cultured human aortic endothelial cells (HAEC) exposed for up to 24 h to either high-shear (13 dyn/cm(2)) steady laminar flow (LF), an established representation of "atheroprotective" flow conditions, or low-shear (<1 dyn/cm(2)), pulsatile, nonsteady, non-unidirectional flow (disturbed flow, DF) that simulates ...

Adhesion under Shear Flow

The 4 integrin, 4 7, plays an important role in recruiting circulating lymphocytes to the gastrointestinal tract, where its ligand mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is preferentially expressed on high endothelial venules (HEVs). Dual antagonists of 4 1 and 4 7, N-(2,6-dichlorobenzoyl)-(L)-4-(2 ,6 -bis-methoxyphenyl)phenylalanine (TR14035) and N-{N-[(3,5-dichlorobenzene)sulfo...

A multi-component parallel-plate flow chamber system for studying the effect of exercise-induced wall shear stress on endothelial cells

BACKGROUND In vivo studies have demonstrated that reasonable exercise training can improve endothelial function. To confirm the key role of wall shear stress induced by exercise on endothelial cells, and to understand how wall shear stress affects the structure and the function of endothelial cells, it is crucial to design and fabricate an in vitro multi-component parallel-plate flow chamber sy...

Erythrocyte deformation under shear flow.